个人简历

学习经历：\r
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1991.03-1994.12:南京农业大学植物保护系 博士；\r
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1987.07-1990.06:南京农业大学植物保护系 硕士；\r
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1980.09-1984.06:南京农业大学植物保护系 本科。\r
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教学及研究经历：\r
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2014.05-- 至今：江苏省农业科学院副院长；\r
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2005.09-2014.05：江苏省农科院食品质量安全与检测研究所所长；\r
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2003.03-2005.09：江苏省农科院食品质量安全与检测研究中心主任；\r
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1999.11-2003.03：江苏省农科院植保所副所长；\r
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1999.01-1999.10：美国俄亥俄州立大学昆虫系高级访问学者；\r
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1995.01-1999.01：江苏省农科院植保所副所长；\r
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1987.07-1994.12：江苏省农科院植保所。

研究领域

""【指导硕、博士生研究方向】\r
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1）农产品危害因子检测与风险评估\r
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2）农产品管控关键技术与体系研究\r
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3）农产品品质与营养\r
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4）农产品安全溯源与智慧管理"""以农产品安全生产全程管控研究为主线，从源头生产到产后加工对农产品安全的机理、规律进行研究，“从农田到餐桌”进行全程风险危害因子的监测、管控技术研究与集成应用；负责植物性农产品中农药残留、致病微生物等为主的危害性检测、安全评价、风险分析、与管控技术体系规范研究、植物性农产品中营养成分分析以及物联网溯源体系等研究。"

近期论文

2019年\r
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1. Xu C, Liu X, Liu Y, Zhang X, Zhang C, Li J, Liu X. High sensitive single chain variable fragment screening from a microcystin-LR immunized mouse phage antibody library and its application in immunoassay. Talanta. 2019 May 15;197:397-405.\r
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2. Zhang L, Li Y, Liang Y, Liang K, Zhang F, Xu T, Wang M, Song H, Liu X, Lu B. Determination of phenolic acid profiles by HPLC-MS in vegetables commonly consumed in China. Food Chem. 2019 Mar 15;276:538-546.\r
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3. Dong S, Liu Y, Zhang X, Xu C, Liu X, Zhang C. Development of an immunochromatographic assay for the specific detection of Bacillus thuringiensis (Bt) Cry1Ab toxin. Anal Biochem. 2019 Feb 15;567:1-7.\r
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2018年\r
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1. Hu X, Zhang X, Zhong J, Liu Y, Zhang C, Xie Y, Lin M, Xu C, Lu L, Zhu Q, Liu X. Expression of Cry1Ac toxin-binding region in Plutella xyllostella cadherin-like receptor and studying their interaction mode by molecular docking and site-directed mutagenesis. Int J Biol Macromol. 2018 May;111:822-831.\r
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2. Dong S, Bo Z, Zhang C, Feng J, Liu X. Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library. Appl Microbiol Biotechnol. 2018 Apr;102(7):3363-3374.\r
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3. Xu C, Yang Y, Liu L, Li J, Liu X, Zhang X, Liu Y, Zhang C, Liu X. Microcystin-LR nanobody screening from an alpaca phage display nanobody library and its expression and application. Ecotoxicol Environ Saf. 2018 Apr 30;151:220-227.\r
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4. Zhong J, Hu X, Zhang X, Liu Y, Xu C, Zhang C, Lin M, Liu X. Broad specificity immunoassay for detection of Bacillus thuringiensis Cry toxins through engineering of a single chain variable fragment with mutagenesis and screening. Int J Biol Macromol. 2018 Feb;107(Pt A):920-928.\r
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2017年\r
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1. Xu C, Zhang C, Zhong J, Hu H, Luo S, Liu X, Zhang X, Liu Y, Liu X. Construction of an immunized rabbit phage display library for selecting high activity against Bacillus thuringiensis Cry1F toxin single-chain antibodies. J Agric Food Chem. 2017 Jul 26;65(29):6016-6022.\r
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2. Jiao L, Liu Y, Zhang X, Liu B, Zhang C, Liu X. Site-saturation mutagenesis library construction and screening for specific broad-spectrum single-domain antibodies against multiple Cry1 toxins. Appl Microbiol Biotechnol. 2017 Aug;101(15):6071-6082.\r
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3. Dong S, Zhang C, Liu Y, Zhang X, Xie Y, Zhong J, Xu C, Liu X. Simultaneous production of monoclonal antibodies against Bacillus thuringiensis (Bt) Cry1 toxins using a mixture immunization. Anal Biochem. 2017 Aug 15;531:60-66.\r
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4. Liu Y, Lin M, Zhang X, Hu X, Lin J, Hao J, He D, Zhang X, Xu C, Zhong J, Xie Y, Zhang C, Liu X. Development of competitive ELISA for the detection of bovine serum albumin using single-chain variable fragments. Anal Biochem. 2017 May 15;525:89-91.\r
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5. Dong S, Zhang X, Liu Y, Zhang C, Xie Y, Zhong J, Xu C, Liu X. Establishment of a sandwich enzyme-linked immunosorbent assay for specific detection of Bacillus thuringiensis (Bt) Cry1Ab toxin utilizing a monoclonal antibody produced with a novel hapten designed with molecular model. Anal Bioanal Chem. 2017 Mar;409(8):1985-1994.\r
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6. Xu C, Liu X, Zhang C, Zhang X, Zhong J, Liu Y, Hu X, Lin M, Liu X. Establishment of a sensitive time-resolved fluoroimmunoassay for detection of Bacillus thuringiensis Cry1Ie toxin based nanobody from a phage display library. Anal Biochem. 2017 Feb 1;518:53-59.\r
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2016年\r
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1. Xu C, Zhang X, Liu X, Liu Y, Hu X, Zhong J, Zhang C, Liu X. Selection and application of broad-specificity human domain antibody for simultaneous detection of Bt Cry toxins. Anal Biochem. 2016 Nov 1;512:70-77.\r
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2. Dong S, Zhang C, Zhang X, Liu Y, Zhong J, Xie Y, Xu C, Ding Y, Zhang L, Liu X. Production and Characterization of Monoclonal Antibody Broadly Recognizing Cry1 Toxins by Use of Designed Polypeptide as Hapten. Anal Chem. 2016 Jul19;88(14):7023-32.\r
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3. Zhao Y, Liang Y, Liu Y, Zhang X, Hu X, Tu S, Wu A, Zhang C, Zhong J, Zhao S, Liu X, Tu K. Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay. Anal Biochem. 2016 Jun 1;502:1-7.
学术社会兼职：南京农业大学及江苏大学博士生导师。